MyMpn Project

Metabolites

To isolate intracellular metabolites, cells were washed thoroughly with PBS containing 0.05% glucose. Cells were lysed and Metabolism was quenched by adding 5-10 ml of -80ºC methanol directly to the culture dishes with attached cells. Lysates were collected, cell debris removed by centrifugation and supernatants were frozen in liquid nitrogen and lyophilyzed. Freeze-dried samples were processed according to analytic requirements (see Maier et al., 2013) and analysed by GC-MS, LC-MS or NMR.

Metabolite name	KEGG ID	Chemical sum formula	Molecular weight	Identified by
(S)-lactate	C00186	C3H6O3	90.08	NMR
3-phospho-D-glycerate	C00197	C3H7O7P	186.06	LC-MS
acetate	C00033	C2H4O2	60.05	NMR
adenine	C00147	C5H5N5	135.14	GC-MS
adenosine	C00212	C10H13N5O4	267.25	GC-MS
ADP	C00008	C10H15N5O10P2	427.21	LC-MS, NMR
ADP-glucose	C00498	C16H25N5O15P2	589.35	LC-MS
alanine	C00041	C3H7NO2	89.1	GC-MS
AMP	C00020	C10H14N5O7P	347.23	LC-MS
arginine	C00062	C6H14N4O2	174.21	GC-MS, NMR
asparagine	C00152	C4H8N2O3	132.12	GC-MS
aspartate	C00049	C4H7NO4	133.11	GC-MS, NMR
ATP	C00002	C10H16N5O13P3	507.19	LC-MS, NMR
CDP	C00112	C9H15N3O11P2	404.19	LC-MS
cholesterol	C00187	C27H46O	386.64	GC-MS
choline	C00114	C5H14NO	104.17	NMR
CMP	C00055	C9H14N3O8P	323.2	LC-MS
CTP	C00063	C9H16N3O14P3	483.16	LC-MS
cysteine	C00097	C3H7NO2S	121.17	GC-MS
cytosine	C00380	C4H5N3O	111.11	GC-MS
D-fructose 1,6-bisphosphate	C00354	C6H14O12P2	340.11	LC-MS, GC-MS
D-glucose 1-phosphate	C00103	C6H13O9P	260.13	LC-MS, GC-MS
D-glucose 6-phosphate	C00092	C6H13O9P	260.13	LC-MS, GC-MS
D-mannitol 1-phosphate	C00644	C6H15O9P	262.15	LC-MS
dADP	C00206	C10H15N5O9P2	411.21	LC-MS
dAMP	C00360	C10H14N5O6P	331.23	LC-MS
dATP	C00131	C10H16N5O12P3	491.19	LC-MS
dGDP	C00361	C10H15N5O10P2	427.21	LC-MS
dGMP	C00362	C10H14N5O7P	347.23	LC-MS
dGTP	C00286	C10H16N5O13P3	507.19	LC-MS
dihydroxyacetone phosphate	C00111	C3H7O6P	170.06	GC-MS
ethanol	C00469	C2H6O	46.07	NMR
FAD	C00016	C27H33N9O15P2	785.56	LC-MS
fatty acids (various)	-	-	0	GC-MS
formate	C00058	CH2O2	46.03	NMR
fructose	C00095	C6H12O6	180.16	GC-MS
fructose 6-phosphate	C00085	C6H13O9P	260.13	GC-MS
fumarate	C00122	C4H4O4	116.07	NMR
glyceraldehyde 3-phosphate	C00118	C3H7O6P	170.06	GC-MS
GDP	C00035	C10H15N5O11P2	443.21	LC-MS
glucose	C00031	C6H12O6	180.16	GC-MS
glutamate	C00025	C5H9NO4	147.13	GC-MS, NMR
glutamine	C00064	C5H10N2O3	146.15	GC-MS
glycerol	C00116	C3H8O3	92.09	GC-MS
glycerol 3-phosphate (sn-Glycerol 3-phosphate)	C00093	C3H9O6P	172.07	LC-MS, GC-MS
glycine	C00037	C2H5NO2	74.06	GC-MS, NMR
GMP	C00144	C10H14N5O8P	363.23	LC-MS
GTP	C00044	C10H16N5O14P3	523.19	LC-MS
guanine	C00242	C5H5N5O	151.14	GC-MS
guanosine	C00387	C10H13N5O5	283.25	GC-MS
histidine	C00135	C6H9N3O2	155.16	GC-MS, NMR
isoleucine	C00407	C6H13NO2	131.17	GC-MS, NMR
leucine	C00123	C6H13NO2	131.17	GC-MS, NMR
lysine	C00047	C6H14N2O2	146.19	GC-MS, NMR
methionine	C00073	C5H11NO2S	149.22	GC-MS, NMR
NAD+	C00003	C21H28N7O14P2	664.44	LC-MS, NMR
NADH	C00004	C21H29N7O14P2	665.45	LC-MS
NADP+	C00006	C21H29N7O17P3	744.42	LC-MS
NADPH	C00005	C21H30N7O17P3	745.43	LC-MS
ornithine	C00077	C5H12N2O2	132.17	NMR
phenylalanine	C00079	C9H11NO2	165.19	GC-MS, NMR
phosphoenolpyruvate	C00074	C3H5O6P	168.04	GC-MS
proline	C00148	C5H9NO2	115.13	GC-MS, NMR
pyruvate	C00022	C3H4O3	88.06	NMR
riboflavin	C00255	C17H20N4O6	376.37	LC-MS
ribose	C00121	C5H10O5	138.12	GC-MS
ribose-5-phosphate	C00199	C5H11O8P	230.11	GC-MS
serine	C00065	C3H7NO3	105.1	GC-MS, NMR
succinate	C00042	C4H6O4	118.09	NMR
thiamine diphosphate	C00068	C12H19N4O7P2S	425.32	LC-MS
threonine	C00188	C4H9NO3	119.12	GC-MS, NMR
thymidine-5'-diphosphate	C00363	C10H16N2O11P2	402.19	LC-MS
thymidine-5'-phosphate	C00364	C10H15N2O8P	322.21	LC-MS
thymidine-5'-triphosphate	C00459	C10H17N2O14P3	482.17	LC-MS
thymine	C00178	C5H6N2O2	126.12	GC-MS
trans-4-hydroxy-L-proline	C03651	C5H9NO3	131.13	NMR
tryptophane	C00078	C11H12N2O2	204.23	GC-MS, NMR
tyrosine	C00082	C9H11NO3	181.19	GC-MS, NMR
UDP	C00015	C9H14N2O12P2	404.16	LC-MS
UDP-D-galactose	C00052	C15H24N2O17P2	566.3	LC-MS, NMR
UDP-D-glucose	C00029	C15H24N2O17P2	566.3	LC-MS, NMR
UMP	C00105	C9H13N2O9P	324.18	LC-MS
uracil	C00106	C4H4N2O2	112.09	GC-MS
uridine	C00299	C9H12N2O6	244.21	GC-MS
UTP	C00075	C9H15N2O15P3	484.14	LC-MS
valine	C00183	C5H11NO2	117.15	GC-MS, NMR